Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
In vitro culture of asexual blood stages of Plasmodium falciparum is essential to study the parasite biology, and several aspects need to be addressed to successfully cultivate the parasites, including the requirements for red blood cells and specific nutrients. Since Trager and Jensen established the technique in 1976, some modifications have been introduced to improve the growth rate and yield. In brief, the method is based on the use of human red blood cells suspended in RPMI-1640 culture medium supplemented with a source of lipids and maintained at 37 °C in a low-oxygen atmosphere. In this protocol, a description of thawing, culturing, and cryopreservation of asexual blood stages of P. falciparum is presented.
Original language | English |
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Title of host publication | Malaria Immunology |
Number of pages | 13 |
Volume | 2470 |
Publisher | Humana Press |
Publication date | 2022 |
Pages | 37-49 |
ISBN (Electronic) | 978-1-0716-2189-9 |
DOIs | |
Publication status | Published - 2022 |
Series | Methods in Molecular Biology |
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Volume | 2470 |
ISSN | 1064-3745 |
Bibliographical note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Culture Media, Erythrocytes/parasitology, Humans, Malaria, Falciparum/parasitology, Plasmodium falciparum
Research areas
ID: 319161462