Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Capsid virus-like particles (cVLPs), assembled from viral coat proteins, are used as therapeutic cargo delivery vehicles as well as molecular scaffolds for display of vaccine antigens. A versatile vaccine platform has been developed based on the Acinetobacter phage AP205 cVLP, which has been shown to significantly improve antigen-specific antibody responses. This modular cVLP platform exploits a split-protein (Tag/Catcher) conjugation system to enable high-density, unidirectional antigen display. Accordingly, protein antigens can be independently expressed and quality-checked prior to conjugation to pre-assembled cVLPs. Here, we describe considerations for the design of vaccine antigens with genetically fused split-protein (Tag or Catcher) binding partners and provide protocols for the expression and purification of corresponding Tag- or Catcher-AP205 cVLPs from E.coli. Finally, we describe a generic protocol for the formulation and quality assessment of experimental/pre-clinical AP205 cVLP-based vaccines.
Original language | English |
---|---|
Title of host publication | Therapeutic Proteins : Methods and Protocols |
Publisher | Humana Press |
Publication date | 2024 |
Pages | 127-141 |
ISBN (Print) | 978-1-0716-3468-4 |
ISBN (Electronic) | 978-1-0716-3469-1 |
DOIs | |
Publication status | Published - 2024 |
Series | Methods in Molecular Biology |
---|---|
Volume | 2720 |
ISSN | 1064-3745 |
Bibliographical note
Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
- AP205, Catcher, cVLP, Density ultracentrifugation, Tag, Virus-like particles
Research areas
ID: 378966950