Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces

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  • Edgar Cristóbal-Lecina
  • Janwa El-Maiss
  • Eduard Figueras
  • Aruna Chandra Singh
  • Sivashankar Krishnamoorthy
  • Østerbye, Thomas
  • César Pascual García
  • David Andreu

In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each amino acid building block with a tert-butyloxycarbonyl (Boc) group that can be removed after each SPPS cycle, in combination with semi-permanent protection of the side chains of trifunctional amino acid residues. Such a protection scheme with a well-proven record of application in conventional, batchwise SPPS has been fine-tuned for optimal performance on CPG and, from there, translated to SPR chips that allow layer-by-layer monitoring of amino acid coupling. Our results validate this acid-modulated synthesis as a feasible approach for producing peptides in high yields and purity on flat glass surfaces, such as those in bio-FETs.

Original languageEnglish
Article number3092
Issue number24
Pages (from-to)1-13
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

    Research areas

  • acid-labile protection scheme, glass surfaces, peptide synthesis, SPR chips

ID: 379714784