A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells. / Sampson, Oliver; Lim, Nicholas; White, Jemima; Vieira, Vinicius; Kløverpris, Henrik; Adland, Emily; Conlon, Chris; Skelly, Donal; Jones, Lucy; Stafford, Lizzie; Jamsen, Anni; Barnes, Ellie; Dunachie, Susie; Frater, John; Klenerman, Paul; Altfeld, Marcus; Goulder, Philip.

In: Journal of Immunological Methods, Vol. 504, 113263, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sampson, O, Lim, N, White, J, Vieira, V, Kløverpris, H, Adland, E, Conlon, C, Skelly, D, Jones, L, Stafford, L, Jamsen, A, Barnes, E, Dunachie, S, Frater, J, Klenerman, P, Altfeld, M & Goulder, P 2022, 'A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells', Journal of Immunological Methods, vol. 504, 113263. https://doi.org/10.1016/j.jim.2022.113263

APA

Sampson, O., Lim, N., White, J., Vieira, V., Kløverpris, H., Adland, E., Conlon, C., Skelly, D., Jones, L., Stafford, L., Jamsen, A., Barnes, E., Dunachie, S., Frater, J., Klenerman, P., Altfeld, M., & Goulder, P. (2022). A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells. Journal of Immunological Methods, 504, [113263]. https://doi.org/10.1016/j.jim.2022.113263

Vancouver

Sampson O, Lim N, White J, Vieira V, Kløverpris H, Adland E et al. A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells. Journal of Immunological Methods. 2022;504. 113263. https://doi.org/10.1016/j.jim.2022.113263

Author

Sampson, Oliver ; Lim, Nicholas ; White, Jemima ; Vieira, Vinicius ; Kløverpris, Henrik ; Adland, Emily ; Conlon, Chris ; Skelly, Donal ; Jones, Lucy ; Stafford, Lizzie ; Jamsen, Anni ; Barnes, Ellie ; Dunachie, Susie ; Frater, John ; Klenerman, Paul ; Altfeld, Marcus ; Goulder, Philip. / A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells. In: Journal of Immunological Methods. 2022 ; Vol. 504.

Bibtex

@article{c842a939cc79450b81a337f996c15590,
title = "A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells",
abstract = "Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell yields and cytokine production after cryopreservation or after even short delays in stimulating freshly isolated cells. We here describe a simple yet robust method for measuring IFN-α production in pDCs that preserves cell activation and cytokine production through immediate stimulation of whole blood and subsequent maintenance at 37 °C.",
keywords = "Immune sex differences, Innate immunity, Interferon alpha, Plasmacytoid dendritic cell, Toll-like receptor 7",
author = "Oliver Sampson and Nicholas Lim and Jemima White and Vinicius Vieira and Henrik Kl{\o}verpris and Emily Adland and Chris Conlon and Donal Skelly and Lucy Jones and Lizzie Stafford and Anni Jamsen and Ellie Barnes and Susie Dunachie and John Frater and Paul Klenerman and Marcus Altfeld and Philip Goulder",
note = "Funding Information: This work was supported by the Wellcome Trust [Grant Number: 108869/Z/15/Z (OS); WT104748MA (PG)] and the University of Oxford Medical Sciences Division COVID19 Fund. Funding bodies had no role in the design, execution, analysis, or submission of the manuscript. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.jim.2022.113263",
language = "English",
volume = "504",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells

AU - Sampson, Oliver

AU - Lim, Nicholas

AU - White, Jemima

AU - Vieira, Vinicius

AU - Kløverpris, Henrik

AU - Adland, Emily

AU - Conlon, Chris

AU - Skelly, Donal

AU - Jones, Lucy

AU - Stafford, Lizzie

AU - Jamsen, Anni

AU - Barnes, Ellie

AU - Dunachie, Susie

AU - Frater, John

AU - Klenerman, Paul

AU - Altfeld, Marcus

AU - Goulder, Philip

N1 - Funding Information: This work was supported by the Wellcome Trust [Grant Number: 108869/Z/15/Z (OS); WT104748MA (PG)] and the University of Oxford Medical Sciences Division COVID19 Fund. Funding bodies had no role in the design, execution, analysis, or submission of the manuscript. Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell yields and cytokine production after cryopreservation or after even short delays in stimulating freshly isolated cells. We here describe a simple yet robust method for measuring IFN-α production in pDCs that preserves cell activation and cytokine production through immediate stimulation of whole blood and subsequent maintenance at 37 °C.

AB - Central to sex differences observed in outcome from infection and vaccination is the innate immune response, and specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), the main producers of IFN-α. Evaluation of IFN-α production by pDCs is therefore critical for studies of innate immune function. However, reliable measurement of pDC IFN-α is hampered by reduced cell yields and cytokine production after cryopreservation or after even short delays in stimulating freshly isolated cells. We here describe a simple yet robust method for measuring IFN-α production in pDCs that preserves cell activation and cytokine production through immediate stimulation of whole blood and subsequent maintenance at 37 °C.

KW - Immune sex differences

KW - Innate immunity

KW - Interferon alpha

KW - Plasmacytoid dendritic cell

KW - Toll-like receptor 7

U2 - 10.1016/j.jim.2022.113263

DO - 10.1016/j.jim.2022.113263

M3 - Journal article

C2 - 35341760

AN - SCOPUS:85127043139

VL - 504

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

M1 - 113263

ER -

ID: 322494865