In vitro neutralization assay using cultured hepatitis C virus
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.
Original language | English |
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Title of host publication | Hepatitis C Virus Protocols |
Number of pages | 7 |
Publisher | Humana Press |
Publication date | 2019 |
Pages | 433-439 |
ISBN (Print) | 978-1-4939-8975-1 |
ISBN (Electronic) | 978-1-4939-8976-8 |
DOIs | |
Publication status | Published - 2019 |
Series | Methods in Molecular Biology |
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Volume | 1911 |
ISSN | 1064-3745 |
- Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine
Research areas
ID: 212851538