In vitro neutralization assay using cultured hepatitis C virus

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

In vitro neutralization assay using cultured hepatitis C virus. / Prentoe, Jannick; Bukh, Jens.

Hepatitis C Virus Protocols. Humana Press, 2019. p. 433-439 (Methods in Molecular Biology, Vol. 1911).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Prentoe, J & Bukh, J 2019, In vitro neutralization assay using cultured hepatitis C virus. in Hepatitis C Virus Protocols. Humana Press, Methods in Molecular Biology, vol. 1911, pp. 433-439. https://doi.org/10.1007/978-1-4939-8976-8_29

APA

Prentoe, J., & Bukh, J. (2019). In vitro neutralization assay using cultured hepatitis C virus. In Hepatitis C Virus Protocols (pp. 433-439). Humana Press. Methods in Molecular Biology Vol. 1911 https://doi.org/10.1007/978-1-4939-8976-8_29

Vancouver

Prentoe J, Bukh J. In vitro neutralization assay using cultured hepatitis C virus. In Hepatitis C Virus Protocols. Humana Press. 2019. p. 433-439. (Methods in Molecular Biology, Vol. 1911). https://doi.org/10.1007/978-1-4939-8976-8_29

Author

Prentoe, Jannick ; Bukh, Jens. / In vitro neutralization assay using cultured hepatitis C virus. Hepatitis C Virus Protocols. Humana Press, 2019. pp. 433-439 (Methods in Molecular Biology, Vol. 1911).

Bibtex

@inbook{08df50c693864735928831a2244c5dcb,
title = "In vitro neutralization assay using cultured hepatitis C virus",
abstract = "The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.",
keywords = "Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine",
author = "Jannick Prentoe and Jens Bukh",
year = "2019",
doi = "10.1007/978-1-4939-8976-8_29",
language = "English",
isbn = "978-1-4939-8975-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "433--439",
booktitle = "Hepatitis C Virus Protocols",
address = "United States",

}

RIS

TY - CHAP

T1 - In vitro neutralization assay using cultured hepatitis C virus

AU - Prentoe, Jannick

AU - Bukh, Jens

PY - 2019

Y1 - 2019

N2 - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

AB - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

KW - Antibodies

KW - HCV

KW - Hepatitis C virus

KW - Immunostaining

KW - Neutralization

KW - Vaccine

U2 - 10.1007/978-1-4939-8976-8_29

DO - 10.1007/978-1-4939-8976-8_29

M3 - Book chapter

C2 - 30593643

AN - SCOPUS:85059235929

SN - 978-1-4939-8975-1

T3 - Methods in Molecular Biology

SP - 433

EP - 439

BT - Hepatitis C Virus Protocols

PB - Humana Press

ER -

ID: 212851538