The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation
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The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation. / von Essen, Marina; Menné, Charlotte; Nielsen, Bodil L; Lauritsen, Jens Peter H; Dietrich, Jes; Andersen, Peter S; Karjalainen, Klaus; Ødum, Niels; Geisler, Carsten.
In: Journal of Immunology, Vol. 168, No. 9, 2002, p. 4519-23.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation
AU - von Essen, Marina
AU - Menné, Charlotte
AU - Nielsen, Bodil L
AU - Lauritsen, Jens Peter H
AU - Dietrich, Jes
AU - Andersen, Peter S
AU - Karjalainen, Klaus
AU - Ødum, Niels
AU - Geisler, Carsten
N1 - Keywords: Amino Acid Motifs; Amino Acid Sequence; Animals; Antigens, CD3; Cell Line; Cells, Cultured; Down-Regulation; Gene Deletion; Humans; Jurkat Cells; Kinetics; Leucine; Ligands; Mice; Molecular Sequence Data; Protein Kinase C; Receptor-CD3 Complex, Antigen, T-Cell; Sequence Homology; T-Lymphocytes
PY - 2002
Y1 - 2002
N2 - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.
AB - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.
M3 - Journal article
C2 - 11970997
VL - 168
SP - 4519
EP - 4523
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -
ID: 8544579