Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
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Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. / Fazli, Mustafa; Rybtke, Morten Levin; Steiner, Elisabeth; Weidel, Elisabeth; Berthelsen, Jens; Groizeleau, Julie Dora Dona; Bin, Wu; Zhi, Boo Zhao; Yaming, Zhang; Kaever, Volkhard; Givskov, Michael; Hartmann, Rolf W; Eberl, Leo; Tolker-Nielsen, Tim.
In: MicrobiologyOpen, Vol. 6, No. 4, e00480, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
AU - Fazli, Mustafa
AU - Rybtke, Morten Levin
AU - Steiner, Elisabeth
AU - Weidel, Elisabeth
AU - Berthelsen, Jens
AU - Groizeleau, Julie Dora Dona
AU - Bin, Wu
AU - Zhi, Boo Zhao
AU - Yaming, Zhang
AU - Kaever, Volkhard
AU - Givskov, Michael
AU - Hartmann, Rolf W
AU - Eberl, Leo
AU - Tolker-Nielsen, Tim
N1 - © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
PY - 2017
Y1 - 2017
N2 - Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
AB - Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
U2 - 10.1002/mbo3.480
DO - 10.1002/mbo3.480
M3 - Journal article
C2 - 28419759
VL - 6
JO - MicrobiologyOpen
JF - MicrobiologyOpen
SN - 2045-8827
IS - 4
M1 - e00480
ER -
ID: 180762191