Quantitative miRNA expression analysis: comparing microarrays with next-generation sequencing
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Quantitative microRNA expression analysis: comparing microarrays with next-generation sequencing.
Final published version, 488 KB, PDF document
Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
Original language | English |
---|---|
Journal | RNA: A publication of the RNA Society |
Volume | 15 |
Issue number | 11 |
Pages (from-to) | 2028-34 |
Number of pages | 6 |
ISSN | 1355-8382 |
DOIs | |
Publication status | Published - 2009 |
Bibliographical note
Keywords: Gene Expression; MicroRNAs; Oligonucleotide Array Sequence Analysis; Reproducibility of Results; Sequence Analysis, RNA
Number of downloads are based on statistics from Google Scholar and www.ku.dk
No data available
ID: 20009726