High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content. / Almblad, Henrik; Rybtke, Morten; Hendiani, Saghar; Andersen, Jens Bo; Givskov, Michael; Tolker-Nielsen, Tim.

In: Microbiology, Vol. 165, No. 3, 03.2019, p. 324-333.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Almblad, H, Rybtke, M, Hendiani, S, Andersen, JB, Givskov, M & Tolker-Nielsen, T 2019, 'High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content', Microbiology, vol. 165, no. 3, pp. 324-333. https://doi.org/10.1099/mic.0.000772

APA

Almblad, H., Rybtke, M., Hendiani, S., Andersen, J. B., Givskov, M., & Tolker-Nielsen, T. (2019). High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content. Microbiology, 165(3), 324-333. https://doi.org/10.1099/mic.0.000772

Vancouver

Almblad H, Rybtke M, Hendiani S, Andersen JB, Givskov M, Tolker-Nielsen T. High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content. Microbiology. 2019 Mar;165(3):324-333. https://doi.org/10.1099/mic.0.000772

Author

Almblad, Henrik ; Rybtke, Morten ; Hendiani, Saghar ; Andersen, Jens Bo ; Givskov, Michael ; Tolker-Nielsen, Tim. / High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content. In: Microbiology. 2019 ; Vol. 165, No. 3. pp. 324-333.

Bibtex

@article{83b3766083dc4bb2a8383fe873f5f2e1,
title = "High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content",
abstract = "The human pathogen Pseudomonas aeruginosa can cause both acute infections and chronic biofilm-based infections. Expression of acute virulence factors is positively regulated by cAMP, whereas biofilm formation is positively regulated by c-di-GMP. We provide evidence that increased levels of cAMP, caused by either a lack of degradation or increased production, inhibit P. aeruginosa biofilm formation. cAMP-mediated inhibition of P. aeruginosa biofilm formation required Vfr, and involved a reduction of the level of c-di-GMP, as well as reduced production of biofilm matrix components. A mutant screen and characterization of defined knockout mutants suggested that a subset of c-di-GMP-degrading phosphodiesterases is involved in cAMP-Vfr-mediated biofilm inhibition in P. aeruginosa.",
author = "Henrik Almblad and Morten Rybtke and Saghar Hendiani and Andersen, {Jens Bo} and Michael Givskov and Tim Tolker-Nielsen",
year = "2019",
month = mar,
doi = "10.1099/mic.0.000772",
language = "English",
volume = "165",
pages = "324--333",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content

AU - Almblad, Henrik

AU - Rybtke, Morten

AU - Hendiani, Saghar

AU - Andersen, Jens Bo

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

PY - 2019/3

Y1 - 2019/3

N2 - The human pathogen Pseudomonas aeruginosa can cause both acute infections and chronic biofilm-based infections. Expression of acute virulence factors is positively regulated by cAMP, whereas biofilm formation is positively regulated by c-di-GMP. We provide evidence that increased levels of cAMP, caused by either a lack of degradation or increased production, inhibit P. aeruginosa biofilm formation. cAMP-mediated inhibition of P. aeruginosa biofilm formation required Vfr, and involved a reduction of the level of c-di-GMP, as well as reduced production of biofilm matrix components. A mutant screen and characterization of defined knockout mutants suggested that a subset of c-di-GMP-degrading phosphodiesterases is involved in cAMP-Vfr-mediated biofilm inhibition in P. aeruginosa.

AB - The human pathogen Pseudomonas aeruginosa can cause both acute infections and chronic biofilm-based infections. Expression of acute virulence factors is positively regulated by cAMP, whereas biofilm formation is positively regulated by c-di-GMP. We provide evidence that increased levels of cAMP, caused by either a lack of degradation or increased production, inhibit P. aeruginosa biofilm formation. cAMP-mediated inhibition of P. aeruginosa biofilm formation required Vfr, and involved a reduction of the level of c-di-GMP, as well as reduced production of biofilm matrix components. A mutant screen and characterization of defined knockout mutants suggested that a subset of c-di-GMP-degrading phosphodiesterases is involved in cAMP-Vfr-mediated biofilm inhibition in P. aeruginosa.

U2 - 10.1099/mic.0.000772

DO - 10.1099/mic.0.000772

M3 - Journal article

C2 - 30663958

VL - 165

SP - 324

EP - 333

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 3

ER -

ID: 216311433