Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells

Department of Immunology and Microbiology

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. / Tibbitt, Christopher Andrew; Stark, Julian Mario; Martens, Liesbet; Ma, Junjie; Mold, Jeff Eron; Deswarte, Kim; Oliynyk, Ganna; Feng, Xiaogang; Lambrecht, Bart Norbert; De Bleser, Pieter; Nylén, Susanne; Hammad, Hamida; Arsenian Henriksson, Marie; Saeys, Yvan; Coquet, Jonathan Marie.

In: Immunity, Vol. 51, No. 1, 16.07.2019, p. 169-184.e5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tibbitt, CA, Stark, JM, Martens, L, Ma, J, Mold, JE, Deswarte, K, Oliynyk, G, Feng, X, Lambrecht, BN, De Bleser, P, Nylén, S, Hammad, H, Arsenian Henriksson, M, Saeys, Y & Coquet, JM 2019, 'Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells', Immunity, vol. 51, no. 1, pp. 169-184.e5. https://doi.org/10.1016/j.immuni.2019.05.014

APA

Tibbitt, C. A., Stark, J. M., Martens, L., Ma, J., Mold, J. E., Deswarte, K., Oliynyk, G., Feng, X., Lambrecht, B. N., De Bleser, P., Nylén, S., Hammad, H., Arsenian Henriksson, M., Saeys, Y., & Coquet, J. M. (2019). Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. Immunity, 51(1), 169-184.e5. https://doi.org/10.1016/j.immuni.2019.05.014

Vancouver

Tibbitt CA, Stark JM, Martens L, Ma J, Mold JE, Deswarte K et al. Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. Immunity. 2019 Jul 16;51(1):169-184.e5. https://doi.org/10.1016/j.immuni.2019.05.014

Author

Tibbitt, Christopher Andrew ; Stark, Julian Mario ; Martens, Liesbet ; Ma, Junjie ; Mold, Jeff Eron ; Deswarte, Kim ; Oliynyk, Ganna ; Feng, Xiaogang ; Lambrecht, Bart Norbert ; De Bleser, Pieter ; Nylén, Susanne ; Hammad, Hamida ; Arsenian Henriksson, Marie ; Saeys, Yvan ; Coquet, Jonathan Marie. / Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. In: Immunity. 2019 ; Vol. 51, No. 1. pp. 169-184.e5.

Bibtex

@article{0f474509639347269da14dceee151658,

title = "Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells",

abstract = "Naive CD4+ T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4+ T, Th1, Th2, regulatory T (Treg) cells, and a CD4+ T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.",

keywords = "Animals, Antigens, Dermatophagoides/immunology, Asthma/immunology, Disease Models, Animal, Humans, Lipid Metabolism/genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Orexin Receptors/genetics, Pyroglyphidae/immunology, Respiratory Hypersensitivity/immunology, Respiratory System/immunology, Sequence Analysis, RNA, Single-Cell Analysis, T-Lymphocyte Subsets/immunology, Th2 Cells/immunology, Transcriptome",

author = "Tibbitt, {Christopher Andrew} and Stark, {Julian Mario} and Liesbet Martens and Junjie Ma and Mold, {Jeff Eron} and Kim Deswarte and Ganna Oliynyk and Xiaogang Feng and Lambrecht, {Bart Norbert} and {De Bleser}, Pieter and Susanne Nyl{\'e}n and Hamida Hammad and {Arsenian Henriksson}, Marie and Yvan Saeys and Coquet, {Jonathan Marie}",

year = "2019",

month = jul,

day = "16",

doi = "10.1016/j.immuni.2019.05.014",

language = "English",

volume = "51",

pages = "169--184.e5",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "1",

}

RIS

TY - JOUR

T1 - Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells

AU - Tibbitt, Christopher Andrew

AU - Stark, Julian Mario

AU - Martens, Liesbet

AU - Ma, Junjie

AU - Mold, Jeff Eron

AU - Deswarte, Kim

AU - Oliynyk, Ganna

AU - Feng, Xiaogang

AU - Lambrecht, Bart Norbert

AU - De Bleser, Pieter

AU - Nylén, Susanne

AU - Hammad, Hamida

AU - Arsenian Henriksson, Marie

AU - Saeys, Yvan

AU - Coquet, Jonathan Marie

PY - 2019/7/16

Y1 - 2019/7/16

N2 - Naive CD4+ T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4+ T, Th1, Th2, regulatory T (Treg) cells, and a CD4+ T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.

AB - Naive CD4+ T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4+ T, Th1, Th2, regulatory T (Treg) cells, and a CD4+ T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.

KW - Animals

KW - Antigens, Dermatophagoides/immunology

KW - Asthma/immunology

KW - Disease Models, Animal

KW - Humans

KW - Lipid Metabolism/genetics

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Orexin Receptors/genetics

KW - Pyroglyphidae/immunology

KW - Respiratory Hypersensitivity/immunology

KW - Respiratory System/immunology

KW - Sequence Analysis, RNA

KW - Single-Cell Analysis

KW - T-Lymphocyte Subsets/immunology

KW - Th2 Cells/immunology

KW - Transcriptome

U2 - 10.1016/j.immuni.2019.05.014

DO - 10.1016/j.immuni.2019.05.014

M3 - Journal article

C2 - 31231035

VL - 51

SP - 169-184.e5

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 1

ER -

ID: 356968648