Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures
Research output: Contribution to journal › Journal article › Research › peer-review
The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.
Original language | English |
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Journal | Acta Pathologica Microbiologica et Immunologica Scandinavica |
Volume | 99 |
Issue number | 2 |
Pages (from-to) | 155-62 |
Number of pages | 7 |
ISSN | 0903-4641 |
Publication status | Published - 1991 |
Bibliographical note
Keywords: Adult; Animals; Antigens, Protozoan; Counterimmunoelectrophoresis; Humans; Immunoblotting; Plasmodium falciparum; Solubility
ID: 6766936