Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum. / Lopez-Perez, Mary; Seidu, Zakaria.

Malaria Immunology. Vol. 2470 Humana Press, 2022. p. 37-49 (Methods in Molecular Biology, Vol. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Lopez-Perez, M & Seidu, Z 2022, Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum. in Malaria Immunology. vol. 2470, Humana Press, Methods in Molecular Biology, vol. 2470, pp. 37-49. https://doi.org/10.1007/978-1-0716-2189-9_5

APA

Lopez-Perez, M., & Seidu, Z. (2022). Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum. In Malaria Immunology (Vol. 2470, pp. 37-49). Humana Press. Methods in Molecular Biology Vol. 2470 https://doi.org/10.1007/978-1-0716-2189-9_5

Vancouver

Lopez-Perez M, Seidu Z. Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum. In Malaria Immunology. Vol. 2470. Humana Press. 2022. p. 37-49. (Methods in Molecular Biology, Vol. 2470). https://doi.org/10.1007/978-1-0716-2189-9_5

Author

Lopez-Perez, Mary ; Seidu, Zakaria. / Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum. Malaria Immunology. Vol. 2470 Humana Press, 2022. pp. 37-49 (Methods in Molecular Biology, Vol. 2470).

Bibtex

@inbook{a94f564e63044315af3ddaee3544bd47,
title = "Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum",
abstract = "In vitro culture of asexual blood stages of Plasmodium falciparum is essential to study the parasite biology, and several aspects need to be addressed to successfully cultivate the parasites, including the requirements for red blood cells and specific nutrients. Since Trager and Jensen established the technique in 1976, some modifications have been introduced to improve the growth rate and yield. In brief, the method is based on the use of human red blood cells suspended in RPMI-1640 culture medium supplemented with a source of lipids and maintained at 37 °C in a low-oxygen atmosphere. In this protocol, a description of thawing, culturing, and cryopreservation of asexual blood stages of P. falciparum is presented.",
keywords = "Culture Media, Erythrocytes/parasitology, Humans, Malaria, Falciparum/parasitology, Plasmodium falciparum",
author = "Mary Lopez-Perez and Zakaria Seidu",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_5",
language = "English",
volume = "2470",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "37--49",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum

AU - Lopez-Perez, Mary

AU - Seidu, Zakaria

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - In vitro culture of asexual blood stages of Plasmodium falciparum is essential to study the parasite biology, and several aspects need to be addressed to successfully cultivate the parasites, including the requirements for red blood cells and specific nutrients. Since Trager and Jensen established the technique in 1976, some modifications have been introduced to improve the growth rate and yield. In brief, the method is based on the use of human red blood cells suspended in RPMI-1640 culture medium supplemented with a source of lipids and maintained at 37 °C in a low-oxygen atmosphere. In this protocol, a description of thawing, culturing, and cryopreservation of asexual blood stages of P. falciparum is presented.

AB - In vitro culture of asexual blood stages of Plasmodium falciparum is essential to study the parasite biology, and several aspects need to be addressed to successfully cultivate the parasites, including the requirements for red blood cells and specific nutrients. Since Trager and Jensen established the technique in 1976, some modifications have been introduced to improve the growth rate and yield. In brief, the method is based on the use of human red blood cells suspended in RPMI-1640 culture medium supplemented with a source of lipids and maintained at 37 °C in a low-oxygen atmosphere. In this protocol, a description of thawing, culturing, and cryopreservation of asexual blood stages of P. falciparum is presented.

KW - Culture Media

KW - Erythrocytes/parasitology

KW - Humans

KW - Malaria, Falciparum/parasitology

KW - Plasmodium falciparum

U2 - 10.1007/978-1-0716-2189-9_5

DO - 10.1007/978-1-0716-2189-9_5

M3 - Book chapter

C2 - 35881337

VL - 2470

T3 - Methods in Molecular Biology

SP - 37

EP - 49

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 319161462