Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection. / Fossat, Nicolas; Lundsgaard, Emma A; Costa, Rui; Rivera-Rangel, Lizandro R; Nielsen, Louise; Mikkelsen, Lotte S; Ramirez, Santseharay; Bukh, Jens; Scheel, Troels K H.

I: Cell Reports, Bind 42, Nr. 4, 112282, 2023.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Fossat, N, Lundsgaard, EA, Costa, R, Rivera-Rangel, LR, Nielsen, L, Mikkelsen, LS, Ramirez, S, Bukh, J & Scheel, TKH 2023, 'Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection', Cell Reports, bind 42, nr. 4, 112282. https://doi.org/10.1016/j.celrep.2023.112282

APA

Fossat, N., Lundsgaard, E. A., Costa, R., Rivera-Rangel, L. R., Nielsen, L., Mikkelsen, L. S., Ramirez, S., Bukh, J., & Scheel, T. K. H. (2023). Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection. Cell Reports, 42(4), [112282]. https://doi.org/10.1016/j.celrep.2023.112282

Vancouver

Fossat N, Lundsgaard EA, Costa R, Rivera-Rangel LR, Nielsen L, Mikkelsen LS o.a. Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection. Cell Reports. 2023;42(4). 112282. https://doi.org/10.1016/j.celrep.2023.112282

Author

Fossat, Nicolas ; Lundsgaard, Emma A ; Costa, Rui ; Rivera-Rangel, Lizandro R ; Nielsen, Louise ; Mikkelsen, Lotte S ; Ramirez, Santseharay ; Bukh, Jens ; Scheel, Troels K H. / Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection. I: Cell Reports. 2023 ; Bind 42, Nr. 4.

Bibtex

@article{abe7a6a3f50c4d65b175d1d1ae7db099,

title = "Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection",

abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had a tremendous impact worldwide. Mapping virus-host interactions is critical to understand disease progression. MicroRNAs (miRNAs) are important RNA regulators, but their interaction with SARS-CoV-2 RNA was not experimentally investigated. Here, using Argonaute (AGO) cross-linking immunoprecipitation combined with RNA proximity ligation (CLEAR-CLIP), we provide unbiased mapping of SARS-CoV-2/miRNA interactions. We identified six main regions on the viral RNA bound primarily by one specific miRNA. Targeted mutagenesis and AGO1-3 knockdown demonstrated that these interactions are not critical for virus production. Moreover, we identified perturbed regulation of cellular miRNA interactions during infection, including non-compensated viral sequestration of the miR-15 family. Transcriptome analysis further showed that mRNAs targeted by this miRNA family are derepressed. This work delineates the interphase between miRNA regulation and SARS-CoV-2 infection and further contributes to deciphering the full molecular interactome of this virus.",

author = "Nicolas Fossat and Lundsgaard, {Emma A} and Rui Costa and Rivera-Rangel, {Lizandro R} and Louise Nielsen and Mikkelsen, {Lotte S} and Santseharay Ramirez and Jens Bukh and Scheel, {Troels K H}",

year = "2023",

doi = "10.1016/j.celrep.2023.112282",

language = "English",

volume = "42",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection

AU - Fossat, Nicolas

AU - Lundsgaard, Emma A

AU - Costa, Rui

AU - Rivera-Rangel, Lizandro R

AU - Nielsen, Louise

AU - Mikkelsen, Lotte S

AU - Ramirez, Santseharay

AU - Bukh, Jens

AU - Scheel, Troels K H

PY - 2023

Y1 - 2023

N2 - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had a tremendous impact worldwide. Mapping virus-host interactions is critical to understand disease progression. MicroRNAs (miRNAs) are important RNA regulators, but their interaction with SARS-CoV-2 RNA was not experimentally investigated. Here, using Argonaute (AGO) cross-linking immunoprecipitation combined with RNA proximity ligation (CLEAR-CLIP), we provide unbiased mapping of SARS-CoV-2/miRNA interactions. We identified six main regions on the viral RNA bound primarily by one specific miRNA. Targeted mutagenesis and AGO1-3 knockdown demonstrated that these interactions are not critical for virus production. Moreover, we identified perturbed regulation of cellular miRNA interactions during infection, including non-compensated viral sequestration of the miR-15 family. Transcriptome analysis further showed that mRNAs targeted by this miRNA family are derepressed. This work delineates the interphase between miRNA regulation and SARS-CoV-2 infection and further contributes to deciphering the full molecular interactome of this virus.

AB - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had a tremendous impact worldwide. Mapping virus-host interactions is critical to understand disease progression. MicroRNAs (miRNAs) are important RNA regulators, but their interaction with SARS-CoV-2 RNA was not experimentally investigated. Here, using Argonaute (AGO) cross-linking immunoprecipitation combined with RNA proximity ligation (CLEAR-CLIP), we provide unbiased mapping of SARS-CoV-2/miRNA interactions. We identified six main regions on the viral RNA bound primarily by one specific miRNA. Targeted mutagenesis and AGO1-3 knockdown demonstrated that these interactions are not critical for virus production. Moreover, we identified perturbed regulation of cellular miRNA interactions during infection, including non-compensated viral sequestration of the miR-15 family. Transcriptome analysis further showed that mRNAs targeted by this miRNA family are derepressed. This work delineates the interphase between miRNA regulation and SARS-CoV-2 infection and further contributes to deciphering the full molecular interactome of this virus.

U2 - 10.1016/j.celrep.2023.112282

DO - 10.1016/j.celrep.2023.112282

M3 - Journal article

C2 - 36961814

VL - 42

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

M1 - 112282

ER -

ID: 340529506