Acid-Modulated Peptide Synthesis for Application on Oxide Biosensor Interfaces
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In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each amino acid building block with a tert-butyloxycarbonyl (Boc) group that can be removed after each SPPS cycle, in combination with semi-permanent protection of the side chains of trifunctional amino acid residues. Such a protection scheme with a well-proven record of application in conventional, batchwise SPPS has been fine-tuned for optimal performance on CPG and, from there, translated to SPR chips that allow layer-by-layer monitoring of amino acid coupling. Our results validate this acid-modulated synthesis as a feasible approach for producing peptides in high yields and purity on flat glass surfaces, such as those in bio-FETs.
Originalsprog | Engelsk |
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Artikelnummer | 3092 |
Tidsskrift | Nanomaterials |
Vol/bind | 13 |
Udgave nummer | 24 |
Sider (fra-til) | 1-13 |
ISSN | 1687-4110 |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
This project was funded by the FET-OPEN program of the European Innovation Council, Grant Number 862539.
Publisher Copyright:
© 2023 by the authors.
ID: 379714784