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Susheel Kumar Singh

Susheel Kumar Singh

Assistant professor

  • Department of Immunology and Microbiology

    Blegdamsvej 3, Building: 07-11-16

    2200 København N

    Phone: +45 35 32 71 75Mobile: +45 52 69 67 90

Member of:

  • GLURP team

I have more than 8 years of research experience in the laboratory. This begins with my PhD from an Indian university, and subsequent post-doctoral work with an emerging biotech industry and the Indian Council of Scientific and Industrial Research (CSIR) laboratory and presently at a premier malaria research center located in Northern Europe.

During my doctoral training at the Rani Durgavati University, Jabalpur, India, I conducted an extensive molecular epidemiological survey using PCR technology for the enterobacterial strains prevalent along the entire length of River Narmada in India on seasonal basis. This study contributed significant understanding of the biodiversity, antibiotics resistance and presence of virulence genes of Shigella spp. in the river Narmada. A report based on this study submitted to the Ministry of Environment and Forest, New Delhi and to Madhya Pradesh Council of Science and Technology, provided an updated antibiotic resistance data and facilitated empiric treatment of local patients suffering from enterobacterial diseases.

After my doctoral studies I worked at the R&D division of a biotech company located in Pune, India, where I spent about two and half years. During this period I successfully cloned and expressed several recombinant proteins from malaria parasite and human cervical cancer under cGMP conditions for use in vaccine trials. For this purpose I used bacterial (Escherchia coli) and yeast (Pichia pastoris) recombinant protein expression platforms. Along with this I have worked to establish an in vitro human papilloma virus (HPV) neutralization bioassay for assessing the efficacy of antibodies generated against L1 immunization.

During my postdoctoral training at the Indian Institute of Integrative Medicine (IIIM), a CSIR laboratory located in Jammu. There I succeeded in cloning and expression of six recombinant malaria enzymes. These include the cyclin dependent kinases and enzymes of the shikimate pathway for establishing biochemical assays for anti-malaria drug discovery and played an important role in establishing a new research laboratory within a short time.

At CMP, I am involved in developing functional bioassays for testing the efficacy of GMZ2, a leading malaria vaccine candidate, currently being tested in Phase IIb clinical trials. I am also playing lead roles in optimizing conditions for expression and purification of correctly folded malaria transmission-blocking vaccine candidate (Pfs48/45) and rational design of particle based antigen delivery for improving the immunogenicity of malaria antigens. I have been involved in optimizing high-throughput bioassay based on flow cytometry for assessing the efficacy of antibodies in malaria vaccine trials which resulted in my publication entitled “Mitochondrial dye CMXRos Red stains live populations of Plasmodium falciparum and could be reliably used to monitor parasite growth inhibition in Antibody-Dependent Cellular Inhibition assay”.

Current research

  • Developing robust and      reliable bioassays for assessing the efficacy of malaria vaccine clinical      trials. This would involve optimizing multiplex immunoassays, western blot      and immunofluorescence assay for detection of parasite antigens and      functional in vitro bioassays for assessing anti-parasite activity      such as GIA and ADCI Assay.
  • Parasitic genotyping      and making antigens for immunization and serological studies.
  • Human pappiloma virus;      VLP technology using as a drug delivery system.
  • Generate models for      screening of drugs.

Selected publications

  1. Published

    Flow cytometric readout based on Mitotracker Red CMXRos staining of live asexual blood stage malarial parasites reliably assesses antibody dependent cellular inhibition

    Jogdand, P. S., Singh, S. K., Christiansen, M., Dziegiel, M. H., Singh, S. & Theisen, M. 2012 In : Malaria Journal. 11, 1, p. 235

    Research output: Contribution to journalJournal article

ID: 33608855