Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB

Research output: Contribution to journalJournal articleResearchpeer-review

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Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. / Fazli, Mustafa; Rybtke, Morten Levin; Steiner, Elisabeth; Weidel, Elisabeth; Berthelsen, Jens; Groizeleau, Julie Dora Dona; Bin, Wu; Zhi, Boo Zhao; Yaming, Zhang; Kaever, Volkhard; Givskov, Michael; Hartmann, Rolf W; Eberl, Leo; Tolker-Nielsen, Tim.

In: MicrobiologyOpen, Vol. 6, No. 4, e00480, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fazli, M, Rybtke, ML, Steiner, E, Weidel, E, Berthelsen, J, Groizeleau, JDD, Bin, W, Zhi, BZ, Yaming, Z, Kaever, V, Givskov, M, Hartmann, RW, Eberl, L & Tolker-Nielsen, T 2017, 'Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB', MicrobiologyOpen, vol. 6, no. 4, e00480. https://doi.org/10.1002/mbo3.480

APA

Fazli, M., Rybtke, M. L., Steiner, E., Weidel, E., Berthelsen, J., Groizeleau, J. D. D., ... Tolker-Nielsen, T. (2017). Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. MicrobiologyOpen, 6(4), [e00480]. https://doi.org/10.1002/mbo3.480

Vancouver

Fazli M, Rybtke ML, Steiner E, Weidel E, Berthelsen J, Groizeleau JDD et al. Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. MicrobiologyOpen. 2017;6(4). e00480. https://doi.org/10.1002/mbo3.480

Author

Fazli, Mustafa ; Rybtke, Morten Levin ; Steiner, Elisabeth ; Weidel, Elisabeth ; Berthelsen, Jens ; Groizeleau, Julie Dora Dona ; Bin, Wu ; Zhi, Boo Zhao ; Yaming, Zhang ; Kaever, Volkhard ; Givskov, Michael ; Hartmann, Rolf W ; Eberl, Leo ; Tolker-Nielsen, Tim. / Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. In: MicrobiologyOpen. 2017 ; Vol. 6, No. 4.

Bibtex

@article{486a2705093143b9852105973b0c7ec8,
title = "Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB",
abstract = "Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.",
author = "Mustafa Fazli and Rybtke, {Morten Levin} and Elisabeth Steiner and Elisabeth Weidel and Jens Berthelsen and Groizeleau, {Julie Dora Dona} and Wu Bin and Zhi, {Boo Zhao} and Zhang Yaming and Volkhard Kaever and Michael Givskov and Hartmann, {Rolf W} and Leo Eberl and Tim Tolker-Nielsen",
note = "{\circledC} 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2017",
doi = "10.1002/mbo3.480",
language = "English",
volume = "6",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "JohnWiley & Sons Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB

AU - Fazli, Mustafa

AU - Rybtke, Morten Levin

AU - Steiner, Elisabeth

AU - Weidel, Elisabeth

AU - Berthelsen, Jens

AU - Groizeleau, Julie Dora Dona

AU - Bin, Wu

AU - Zhi, Boo Zhao

AU - Yaming, Zhang

AU - Kaever, Volkhard

AU - Givskov, Michael

AU - Hartmann, Rolf W

AU - Eberl, Leo

AU - Tolker-Nielsen, Tim

N1 - © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

PY - 2017

Y1 - 2017

N2 - Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.

AB - Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.

U2 - 10.1002/mbo3.480

DO - 10.1002/mbo3.480

M3 - Journal article

VL - 6

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 4

M1 - e00480

ER -

ID: 180762191