Claudia Nastasi

Claudia Nastasi

Assistant professor

 Curriculum Vitae

CLAUDIA NASTASI, PhD

Date of Birth: 4th October 1986

Nationality: Italian

e-mail: cnastasi@sund.ku.dk,

Mobile +45 31156544

Education

 

Jan 2012 – Apr 2015 

PhD degree – University of Bologna 

Enrolled at the XXVII ciclo of “Cellular and Molecular Biology” PhD school

 

September 2012

Passed the Italian government exam and licensed as professional qualified Biologist  

Final grade: 195/200

  

Nov 2009 - Jul 2012

Master degree in Cellular and Molecular Biology (LM6)University of Palermo Final grade: 110/110 cum laude and honor

 

Sept 2005 - Oct 2009

Bachelor degree in Scienze biologicheUniversity of Palermo

Final grade: 110/110 cum laude.

 

 

Research Experience

 

Apr 2015 – to present

Post-doctoral researcher

University of Copenhagen, Department Immunology and Microbiology (ISIM)

  • Heads of the group: Niels Ødum and Anders Woetmann

 

Apr 2015 – Oct 2015

Internship- Post doc researcher

Herlev Hospital, CCIT, Department of Hematology, Denmark

  •  Head of the group: Mads Hald Andersen

 Jan 2014- April 2015

 Guest- PhD student 

 University of Copenhagen, Department of Immunology and Microbiology

 Supervisors: Prof. Niels Ødum

 

Jan 2012 – Apr 2015 

PhD Student at “Cellular and Molecular biology” School

 University of Bologna, Department of Pharmacy and Biotechnology FaBiT, Bologna, Italy

  • Workplace: Oncology and haematology Lalla Seragnoli laboratory - Pediatric Unit- S.Orsola Malpighi Hospital, Bologna- Italy. 
  • Tutor and advisor: Prof. Andrea Pession.In Collaboration with: Prof. Patrizia Brigidiand Ass. Prof. Marco Candela- Department of Pharmacology. 
  • PhD thesis: "Interactions between the gut microbiota, short-chain fatty acids and the immune system in pediatric patients undergoing allogeneic Hematopoietic Stem Cell Transplantation". 

 

Oct 2010 - Jul 2011 

Master thesis at Genetics of Microorganisms lab, University of Palermo. Thesis project: “Overexpression of the gene PEPAmyof Amycolatopsis balhimycina in E.coli”.

  • Tutor and advisor: Prof. Anna Maria Puglia- University of Palermo 

 

 May 2009 - Jul 2009 

Bachelor thesis at Genetics lab, University of Palermo. Thesis project: “Optimal conditions for the expression of the gene rho in E.coli” 

  • Tutor and Advisor: Prof. Mario La Farina- University of Palermo

 

Skills & background

Background knowledge 

Human Immunology: T-Cell Culture, inflammation, immune regulation, antigen Presentation, macrophage and dendritic cells differentiation. General microbiology, interaction between microbes and the immune system, mucosal immunity, gut microbiome. Cancer development and immunotherapy. 

Technical skills

Molecular biology(DNA/RNA isolation, qPCRs, protein isolation, Western-blot, ELISA and Elispot assays); Cellular Biology(CD8 killing assay, LDH cytotoxicity assay, proliferation assay, cell culture experience (primary cells and cancer cell lines). PBMCs isolationvia ficoll gradient, primary cells isolation from PBMCs, dendritic cells and macrophage differentiation, co-culture of human DC - T cells. Advanced user of flow cytometry(BD machines used: Canto, LSR, Fortessa 3 and 5 lasers). Data analysis by basic statistical methods.

Computer skills

Macintosh, Microsoft, Office, GraphPad Prism, Photoshop, Illustrator, Flowjo and DIVA softwares for flow cytometry data. 

Languages

English (C1), Danish (B1), Italian (mother-tongue)

 

Teaching Experience

 

Lecturer at the Master’s courses Advanced Immunology and Human Immunology.

 Innate Immunity (year 2018), Mucosal Immunity (years 2016-2018), Immunometabolism (2017-2018)

 

Lecturer at the Faculty of Medicine for the “Innate Immunity” (year 2018).

 

 

Awards & Grants

Maj 2018 

BIRD (Best young Italian Researcher in Danmark) award 2018 given by Ministero degli Affari Esteri e della Cooperazione Internazionale- Italian Embassy in Danmark. 

January 2017

Dyssegaards Fonds’ grant (30.000dkk) for the attendance to national and international conferences and advanced courses.

Nov 2013

Marco Polo Fellowship for abroad stage (Jan 2014- Aug 2014): "Gut microbiome immunomodulatory activity in pediatrics patients who underwent allogeneic haematopoietic stem cell transplantation (aHSCT) with or without acute Graft-versus Host disease (aGVHD)". 

August 2013

University of Bologna fellowship (august 2013-august 2014): "Functional analysis and gut microbiota's characterization related to general recovery of the eubiotic homeostasis in pediatrics patients underwent to hematopoietic stem cell transplantation".

Publications

 
  • Buus TB, Willerslev-Olsen A, Fredholm S, Blümel E, Nastasi C et al.- Single cell heterogeneity in Sezary syndrome. Blood Adv. 2018 Aug 28;2(16):2115-2126. doi:10.1182/bloodadvances.2018022608
  • Fredholm S, Willerslev-Olsen A, Met Ö, Kubat L, Gluud M, Mathiasen SL, Friese C, Blümel E, Petersen DL, Hu T, Nastasi C, Lindahl LM, Buus TB, Krejsgaard T, Wasik MA, Kopp KL, Koralov SB, Persson JL, Bonefeld CM, Geisler C, Woetmann A, Iversen L, Becker JC, Odum N. -Special AT rich-binding1 protein (SATB1) in malignant T cells. J Invest Dermatol. 2018 May 8. pii: S0022 202X(18)31956-0. doi: 10.1016/j.jid.2018.03.1526. 
  • Woetmann A, Alhede M, Dabelsteen S, Bjarnsholt T, Rybtke M, Nastasi C, Krejsgaard T, Andersen MH, Bonefeld CM, Geisler C, Givskov M, Odum N -Interleukin-26 (IL-26) is a novel anti-microbial peptide produced by T cells in response to staphylococcal enterotoxin. Oncotarget. 2018 Apr 13;9(28):19481-19489. doi: 10.18632/oncotarget.24603. eCollection 2018 Apr 13.
  • Lise M Lindahl, Simon Fredholm, Claudine Joseph, Boye Schnack Nielsen, Lars Jønson, Andreas Willerslev-Olsen, Maria Gluud, Edda Blümel, David L Petersen, Nina Sibbesen, Tengpeng Hu, Claudia Nastasi, Thorbjørn Krejsgaard, Ditte Jæhger, Jenny L Persson, Nigel P. Mongan, Mariusz A Wasik, Ivan V Litvinov, Denis Sasseville, Sergei B Koralov, Charlotte M Bonefeld, Carsten Geisler, Anders Woetmann, Elisabeth Ralfkiaer, Lars Iversen, Niels Odum: STAT5 induces miR-21 expression in cutaneous T cell lymphoma. Oncotarget 06/2016; 7(29). DOI:10.18632/oncotarget.10160
  • Lisa Del Bel Belluz, Riccardo Guidi, Ioannis S. Pateras, Laura Levi, Boris Mihaljevic, Syed Fazle Rouf, Marie Wrande, Marco Candela, Silvia Turroni, Claudia Nastasi, Clarissa Consolandi, Clelia Peano, Toma Tebaldi, Gabriella Viero, Vassilis G. Gorgoulis, Thorbjørn Krejsgaard, Mikael Rhen, Teresa Frisan: The Typhoid Toxin Promotes Host Survival and the Establishment of a Persistent Asymptomatic Infection. PLoS Pathogens 04/2016; 12(4). DOI:10.1371/journal.ppat.1005528
  • Simon Fredholm, Ivan Livinov, Nigel P. Mongan, Sarah Schiele, Andreas Willerslev-Olsen, David Leander Petersen, Thorbjørn Krejsgaard, Nina Sibbesen, Claudia Nastasi, Charlotte M. Bonefeld, Jenny L. Persson, Per Thor Straten, Mads Hald Andersen, Sergei B. Koralov, Mariusz M Wasik, Carsten Geisler, Denis Sasseville, Anders Woetmann, Niels Ødum- The Expression of IL-21 Is Promoted by MEKK4 in Malignant T Cells and Associated with Increased Progression Risk in Cutaneous T-Cell Lymphoma. Journal of Investigative Dermatology 01/2016; 136(4). DOI:10.1016/j.jid.2015.12.03 
  • Andreas Willerslev-Olsen, Thorbjørn Krejsgaard, Lise M Lindahl, Ivan V Litvinov, Simon Fredholm, David L Petersen, Claudia Nastasi, Robert Gniadecki, Nigel P Mongan, Denis Sasseville, Mariusz A Wasik, Charlotte M Bonefeld, Carsten Geisler, Anders Woetmann, Lars Iversen, Mogens Kilian, Sergei Koralov, Niels Odum- Staphylococcus aureus enterotoxin A (SEA) stimulates STAT3 activation and IL-17 expression in cutaneous T-cell lymphoma. Blood 01/2016; 127(10). DOI:10.1182/blood-2015-08-66235.
  • Claudia Nastasi, Marco Candela, Charlotte Menné Bonefeld, Carsten Geisler, Morten Hansen, Thorbjørn Krejsgaard, Elena Biagi, Mads Hald Andersen, Patrizia Brigidi, Niels Ødum, Thomas Litman, Anders Woetmann: The effect of short-chain fatty acids on human monocyte-derived dendritic cells. Scientific Reports 11/2015; 5. DOI:10.1038/srep16148
  • Nina A Sibbesen, Katharina L Kopp, Ivan V Litvinov, Lars Jønson, Andreas Willerslev-Olsen, Simon Fredholm, David L Petersen, Claudia Nastasi, Thorbjørn Krejsgaard, Lise M Lindahl, Robert Gniadecki, Nigel P Mongan, Denis Sasseville, Mariusz A Wasik, Lars Iversen, Charlotte M Bonefeld, Carsten Geisler, Anders Woetmann, Niels Odum- Jak3, STAT3, and STAT5 inhibit expression of miR-22, a novel tumor suppressor microRNA, in cutaneous T-Cell lymphoma. Oncotarget 05/2015; 6(24). DOI:10.18632/oncotarget.4111
  • E Biagi, D Zama, C Nastasi, C Consolandi, J Fiori, S Rampelli, S Turroni, M Centanni, M Severgnini, C Peano, G de Bellis, G Basaglia, R Gotti, R Masetti, A Pession, P Brigidi, M Candela: Gut microbiota trajectory in pediatric patients undergoing hematopoietic SCT. Bone marrow transplantation 04/2015; 50(7). DOI:10.1038/bmt.2015.16
  • Ieva Bagdonaite, Hans H Wandall, Ivan V Litvinov, Claudia Nastasi, Jürgen C Becker, Sally Dabelsteen, Carsten Geisler, Charlotte M Bonefeld, Qian Zhang, Mariusz A Wasik, Youwen Zhou, Denis Sasseville, Niels Ødum, Anders Woetmann: Ectopic expression of a novel CD22 splice-variant regulates survival and proliferation in malignant T cells from cutaneous T cell lymphoma (CTCL) patients. Oncotarget 03/2015; 6(17). DOI:10.18632/oncotarget.3720

Conferences´ contributions

  • Keystone symposium “Integrating metabolism and immunity” May 29—June 2, 2017, Royal Dublin Society • Dublin, Ireland.
  • The Copenhagen Workshop on Cutaneous T Cell Lymphoma - Copenhagen 22nd - 25th  August 2016
  • Midwinter congress - Seedfeld, Tyrol 17th – 21th   January 2015.
  • AIEOP IN LAB, 8-9th  October 2013, Pavia, Italy.
  • FEMS Microbiology congress 2013 – 21st  -25th  July 2013 - Liepzig, Germany;
  • XXXVIII Congresso Nazionale AIEOP 9-11th  June 2013 - Rome, Italy;
  • The microbiota and the immunity in human diasease Symposium - Children's Hospital Bambino Gesù – 3rd -4th  May 2013 - Rome, Italy;
  • EBMT 2013, 9-11th  April 2013 – London;
  • SIMGBM 29° National meeting”- Pisa 21st -23th September 2011;
  • European Summer School of Biological chemistry and Advanced Biotechnology-  5-7th  September 2011 – Berlin.

 

 

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