Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems. / Fredsgaard, Laurits; Goksøyr, Louise; Thrane, Susan; Aves, Kara Lee; Theander, Thor G.; Sander, Adam F.

I: Vaccines, Bind 9, Nr. 6, 539, 06.2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Fredsgaard, L, Goksøyr, L, Thrane, S, Aves, KL, Theander, TG & Sander, AF 2021, 'Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems', Vaccines, bind 9, nr. 6, 539. https://doi.org/10.3390/vaccines9060539

APA

Fredsgaard, L., Goksøyr, L., Thrane, S., Aves, K. L., Theander, T. G., & Sander, A. F. (2021). Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems. Vaccines, 9(6), [539]. https://doi.org/10.3390/vaccines9060539

Vancouver

Fredsgaard L, Goksøyr L, Thrane S, Aves KL, Theander TG, Sander AF. Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems. Vaccines. 2021 jun.;9(6). 539. https://doi.org/10.3390/vaccines9060539

Author

Fredsgaard, Laurits ; Goksøyr, Louise ; Thrane, Susan ; Aves, Kara Lee ; Theander, Thor G. ; Sander, Adam F. / Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems. I: Vaccines. 2021 ; Bind 9, Nr. 6.

Bibtex

@article{ba2c7ec020784bc58abfa5107f6f9b49,

title = "Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems",

abstract = "Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains unknown to what extent the employed cVLP backbone and conjugation system may influence the immune response elicited against the displayed antigen. Here, we performed a head-to-head comparison of antigen-specific IgG responses elicited by modular cVLP-vaccines differing by their employed cVLP backbone or conjugation system, respectively. Covalent antigen conjugation (i.e., employing the SpyTag/SpyCatcher system) resulted in significantly higher antigen-specific IgG titers compared to when using affinity-based conjugation (i.e., using biotin/streptavidin). The cVLP backbone also influenced the antigen-specific IgG response. Specifically, vaccines based on the bacteriophage AP205 cVLP elicited significantly higher antigen-specific IgG compared to corresponding vaccines using the human papillomavirus major capsid protein (HPV L1) cVLP. In addition, the AP205 cVLP platform mediated induction of antigen-specific IgG with a different subclass profile (i.e., higher IgG2a and IgG2b) compared to HPV L1 cVLP. These results demonstrate that the cVLP backbone and conjugation system can individually affect the IgG response elicited against a displayed antigen. These data will aid the understanding and process of tailoring modular cVLP vaccines to achieve improved immune responses.",

keywords = "Conjugation system, Modular vaccine platform, Vaccine, Vaccine design, Virus-like particle",

author = "Laurits Fredsgaard and Louise Goks{\o}yr and Susan Thrane and Aves, {Kara Lee} and Theander, {Thor G.} and Sander, {Adam F.}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jun,

doi = "10.3390/vaccines9060539",

language = "English",

volume = "9",

journal = "Vaccines",

issn = "2076-393X",

publisher = "MDPI AG",

number = "6",

}

RIS

TY - JOUR

T1 - Head-to-head comparison of modular vaccines developed using different capsid virus-like particle backbones and antigen conjugation systems

AU - Fredsgaard, Laurits

AU - Goksøyr, Louise

AU - Thrane, Susan

AU - Aves, Kara Lee

AU - Theander, Thor G.

AU - Sander, Adam F.

PY - 2021/6

Y1 - 2021/6

N2 - Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains unknown to what extent the employed cVLP backbone and conjugation system may influence the immune response elicited against the displayed antigen. Here, we performed a head-to-head comparison of antigen-specific IgG responses elicited by modular cVLP-vaccines differing by their employed cVLP backbone or conjugation system, respectively. Covalent antigen conjugation (i.e., employing the SpyTag/SpyCatcher system) resulted in significantly higher antigen-specific IgG titers compared to when using affinity-based conjugation (i.e., using biotin/streptavidin). The cVLP backbone also influenced the antigen-specific IgG response. Specifically, vaccines based on the bacteriophage AP205 cVLP elicited significantly higher antigen-specific IgG compared to corresponding vaccines using the human papillomavirus major capsid protein (HPV L1) cVLP. In addition, the AP205 cVLP platform mediated induction of antigen-specific IgG with a different subclass profile (i.e., higher IgG2a and IgG2b) compared to HPV L1 cVLP. These results demonstrate that the cVLP backbone and conjugation system can individually affect the IgG response elicited against a displayed antigen. These data will aid the understanding and process of tailoring modular cVLP vaccines to achieve improved immune responses.

AB - Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains unknown to what extent the employed cVLP backbone and conjugation system may influence the immune response elicited against the displayed antigen. Here, we performed a head-to-head comparison of antigen-specific IgG responses elicited by modular cVLP-vaccines differing by their employed cVLP backbone or conjugation system, respectively. Covalent antigen conjugation (i.e., employing the SpyTag/SpyCatcher system) resulted in significantly higher antigen-specific IgG titers compared to when using affinity-based conjugation (i.e., using biotin/streptavidin). The cVLP backbone also influenced the antigen-specific IgG response. Specifically, vaccines based on the bacteriophage AP205 cVLP elicited significantly higher antigen-specific IgG compared to corresponding vaccines using the human papillomavirus major capsid protein (HPV L1) cVLP. In addition, the AP205 cVLP platform mediated induction of antigen-specific IgG with a different subclass profile (i.e., higher IgG2a and IgG2b) compared to HPV L1 cVLP. These results demonstrate that the cVLP backbone and conjugation system can individually affect the IgG response elicited against a displayed antigen. These data will aid the understanding and process of tailoring modular cVLP vaccines to achieve improved immune responses.

KW - Conjugation system

KW - Modular vaccine platform

KW - Vaccine

KW - Vaccine design

KW - Virus-like particle

U2 - 10.3390/vaccines9060539

DO - 10.3390/vaccines9060539

M3 - Journal article

C2 - 34063871

AN - SCOPUS:85107236245

VL - 9

JO - Vaccines

JF - Vaccines

SN - 2076-393X

IS - 6

M1 - 539

ER -

ID: 272067930